Construct: shRNA TRCN0000290050

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001046.2-3705s21c1
DNA Barcode:
GCTCTCTACATGGCATGGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC12A2 (6558)
Gene Description:
solute carrier family 12 member 2
Transcript:
RefSeq NM_001046.2 (NON-CURRENT)
Match location:
Position 3705 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290050)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCTACATGGCATGGTTA
Hairpin Sequence:
5'-CCGG-GCTCTCTACATGGCATGGTTA-CTCGAG-TAACCATGCCATGTAGAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCTACATGGCATGGTTACTCGAGTAACCATGCCATGTAGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCTACATGGCATGGTTACTCGAGTAACCATGCCATGTAGAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000042944