Construct: shRNA TRCN0000290158

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002615.4-1100s21c1
DNA Barcode:
CCCAAGCTGAAGCTGAGTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SERPINF1 (5176)
Gene Description:
serpin family F member 1
Transcript:
RefSeq NM_002615.4 (NON-CURRENT)
Match location:
Position 1100 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290158)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGCTGAAGCTGAGTTAT
Hairpin Sequence:
5'-CCGG-CCCAAGCTGAAGCTGAGTTAT-CTCGAG-ATAACTCAGCTTCAGCTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGCTGAAGCTGAGTTATCTCGAGATAACTCAGCTTCAGCTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGCTGAAGCTGAGTTATCTCGAGATAACTCAGCTTCAGCTTGGG-3'

Other clones with same target sequence:

  1. TRCN0000052293