Construct: shRNA TRCN0000290199

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003628.3-1482s21c1
DNA Barcode:
GACTTGCACATTACTCCTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PKP4 (8502)
Gene Description:
plakophilin 4
Transcript:
RefSeq NM_003628.3 (NON-CURRENT)
Match location:
Position 1482 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290199)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTTGCACATTACTCCTATA
Hairpin Sequence:
5'-CCGG-GACTTGCACATTACTCCTATA-CTCGAG-TATAGGAGTAATGTGCAAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTTGCACATTACTCCTATACTCGAGTATAGGAGTAATGTGCAAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTTGCACATTACTCCTATACTCGAGTATAGGAGTAATGTGCAAGTC-3'

Other clones with same target sequence:

  1. TRCN0000123183