Construct: shRNA TRCN0000290236

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003128.2-3010s21c1
DNA Barcode:
CCAAGTGAGAAGGAAATCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPTBN1 (6711)
Gene Description:
spectrin beta, non-erythrocytic 1
Transcript:
RefSeq NM_003128.2 (NON-CURRENT)
Match location:
Position 3010 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290236)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGTGAGAAGGAAATCAAA
Hairpin Sequence:
5'-CCGG-CCAAGTGAGAAGGAAATCAAA-CTCGAG-TTTGATTTCCTTCTCACTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGTGAGAAGGAAATCAAACTCGAGTTTGATTTCCTTCTCACTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGTGAGAAGGAAATCAAACTCGAGTTTGATTTCCTTCTCACTTGG-3'

Other clones with same target sequence:

  1. TRCN0000116826