Construct: shRNA TRCN0000290249

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001745.2-340s21c1
DNA Barcode:
CCTTCCGTTTCAAAGCGAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMLG (819)
Gene Description:
calcium modulating ligand
Transcript:
RefSeq NM_001745.2 (NON-CURRENT)
Match location:
Position 340 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290249)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCCGTTTCAAAGCGAGTA
Hairpin Sequence:
5'-CCGG-CCTTCCGTTTCAAAGCGAGTA-CTCGAG-TACTCGCTTTGAAACGGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCCGTTTCAAAGCGAGTACTCGAGTACTCGCTTTGAAACGGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCCGTTTCAAAGCGAGTACTCGAGTACTCGCTTTGAAACGGAAGG-3'

Other clones with same target sequence:

  1. TRCN0000055992