Construct: shRNA TRCN0000290393

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000158.3-2499s21c1
DNA Barcode:
CCAAGCCATCAAGTGTCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GBE1 (2632)
Gene Description:
1,4-alpha-glucan branching enzyme 1
Transcript:
RefSeq NM_000158.3 (NON-CURRENT)
Match location:
Position 2499 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290393)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGCCATCAAGTGTCTGAA
Hairpin Sequence:
5'-CCGG-CCAAGCCATCAAGTGTCTGAA-CTCGAG-TTCAGACACTTGATGGCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGCCATCAAGTGTCTGAACTCGAGTTCAGACACTTGATGGCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGCCATCAAGTGTCTGAACTCGAGTTCAGACACTTGATGGCTTGG-3'

Other clones with same target sequence:

  1. TRCN0000083098