Construct: shRNA TRCN0000290465
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001344.2-472s21c1
- DNA Barcode:
- CTCTTTGAATTTCCTGGATAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DAD1 (1603)
- Gene Description:
- defender against cell death 1
- Transcript:
- RefSeq NM_001344.2 (NON-CURRENT)
- Match location:
- Position 472 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000290465)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTCTTTGAATTTCCTGGATAA
- Hairpin Sequence:
-
5'-CCGG-CTCTTTGAATTTCCTGGATAA-CTCGAG-TTATCCAGGAAATTCAAAGAG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTCTTTGAATTTCCTGGATAACTCGAGTTATCCAGGAAATTCAAAGAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTCTTTGAATTTCCTGGATAACTCGAGTTATCCAGGAAATTCAAAGAG-3'
Other clones with same target sequence:
-
TRCN0000083038
