Construct: shRNA TRCN0000290468

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014452.3-2221s21c1
DNA Barcode:
GCAGCCTATCTTTGATGACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNFRSF21 (27242)
Gene Description:
TNF receptor superfamily member 21
Transcript:
RefSeq NM_014452.3 (NON-CURRENT)
Match location:
Position 2221 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290468)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCCTATCTTTGATGACAT
Hairpin Sequence:
5'-CCGG-GCAGCCTATCTTTGATGACAT-CTCGAG-ATGTCATCAAAGATAGGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCCTATCTTTGATGACATCTCGAGATGTCATCAAAGATAGGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCCTATCTTTGATGACATCTCGAGATGTCATCAAAGATAGGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000059133