Construct: shRNA TRCN0000290620

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017836.3-475s21c1
DNA Barcode:
CGGCATGCTTCTGGACTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC41A3 (54946)
Gene Description:
solute carrier family 41 member 3
Transcript:
RefSeq NM_017836.3 (CURRENT)
Match location:
Position 475 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290620)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCATGCTTCTGGACTATTT
Hairpin Sequence:
5'-CCGG-CGGCATGCTTCTGGACTATTT-CTCGAG-AAATAGTCCAGAAGCATGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCATGCTTCTGGACTATTTCTCGAGAAATAGTCCAGAAGCATGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCATGCTTCTGGACTATTTCTCGAGAAATAGTCCAGAAGCATGCCG-3'

Other clones with same target sequence:

  1. TRCN0000044672