Construct: shRNA TRCN0000290648

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000175.2-838s21c1
DNA Barcode:
CCTGTCTACTAACACAACCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPI (2821)
Gene Description:
glucose-6-phosphate isomerase
Transcript:
RefSeq NM_000175.2 (NON-CURRENT)
Match location:
Position 838 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290648)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCTACTAACACAACCAA
Hairpin Sequence:
5'-CCGG-CCTGTCTACTAACACAACCAA-CTCGAG-TTGGTTGTGTTAGTAGACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCTACTAACACAACCAACTCGAGTTGGTTGTGTTAGTAGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCTACTAACACAACCAACTCGAGTTGGTTGTGTTAGTAGACAGG-3'

Other clones with same target sequence:

  1. TRCN0000049151