Construct: shRNA TRCN0000290713

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003132.2-638s21c1
DNA Barcode:
AGTCTCTTCAAGGAGTCCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRM (6723)
Gene Description:
spermidine synthase
Transcript:
RefSeq NM_003132.2 (NON-CURRENT)
Match location:
Position 638 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290713)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCTCTTCAAGGAGTCCTAT
Hairpin Sequence:
5'-CCGG-AGTCTCTTCAAGGAGTCCTAT-CTCGAG-ATAGGACTCCTTGAAGAGACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCTCTTCAAGGAGTCCTATCTCGAGATAGGACTCCTTGAAGAGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCTCTTCAAGGAGTCCTATCTCGAGATAGGACTCCTTGAAGAGACT-3'

Other clones with same target sequence:

  1. TRCN0000045728