Construct: shRNA TRCN0000290810

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004753.4-1344s21c1
DNA Barcode:
ACCTGCATGAACACTTTCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DHRS3 (9249)
Gene Description:
dehydrogenase/reductase 3
Transcript:
RefSeq NM_004753.4 (NON-CURRENT)
Match location:
Position 1344 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290810)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTGCATGAACACTTTCAAA
Hairpin Sequence:
5'-CCGG-ACCTGCATGAACACTTTCAAA-CTCGAG-TTTGAAAGTGTTCATGCAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTGCATGAACACTTTCAAACTCGAGTTTGAAAGTGTTCATGCAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTGCATGAACACTTTCAAACTCGAGTTTGAAAGTGTTCATGCAGGT-3'

Other clones with same target sequence:

  1. TRCN0000036532