Construct: shRNA TRCN0000290811

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004753.4-677s21c1
DNA Barcode:
CCGGACTGAGAAATGCCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DHRS3 (9249)
Gene Description:
dehydrogenase/reductase 3
Transcript:
RefSeq NM_004753.4 (NON-CURRENT)
Match location:
Position 677 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290811)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGACTGAGAAATGCCTGAA
Hairpin Sequence:
5'-CCGG-CCGGACTGAGAAATGCCTGAA-CTCGAG-TTCAGGCATTTCTCAGTCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGACTGAGAAATGCCTGAACTCGAGTTCAGGCATTTCTCAGTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGACTGAGAAATGCCTGAACTCGAGTTCAGGCATTTCTCAGTCCGG-3'

Other clones with same target sequence:

  1. TRCN0000036531