Construct: shRNA TRCN0000290825

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005640.1-1197s21c1
DNA Barcode:
GCCCAATCTTAAAGCAGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAF4B (6875)
Gene Description:
TATA-box binding protein associated factor 4b
Transcript:
RefSeq NM_005640.1 (NON-CURRENT)
Match location:
Position 1197 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290825)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAATCTTAAAGCAGAGAA
Hairpin Sequence:
5'-CCGG-GCCCAATCTTAAAGCAGAGAA-CTCGAG-TTCTCTGCTTTAAGATTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAATCTTAAAGCAGAGAACTCGAGTTCTCTGCTTTAAGATTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAATCTTAAAGCAGAGAACTCGAGTTCTCTGCTTTAAGATTGGGC-3'

Other clones with same target sequence:

  1. TRCN0000053148