Construct: shRNA TRCN0000290900

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016426.5-1544s21c1
DNA Barcode:
GCCTACTCCTACAAATCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GTSE1 (51512)
Gene Description:
G2 and S-phase expressed 1
Transcript:
RefSeq NM_016426.5 (NON-CURRENT)
Match location:
Position 1544 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290900)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTACTCCTACAAATCAATT
Hairpin Sequence:
5'-CCGG-GCCTACTCCTACAAATCAATT-CTCGAG-AATTGATTTGTAGGAGTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTACTCCTACAAATCAATTCTCGAGAATTGATTTGTAGGAGTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTACTCCTACAAATCAATTCTCGAGAATTGATTTGTAGGAGTAGGC-3'

Other clones with same target sequence:

  1. TRCN0000113878