Construct: shRNA TRCN0000290966

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022551.2-166s21c1
DNA Barcode:
GCTCATGTGGTGTTGAGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS18 (6222)
Gene Description:
ribosomal protein S18
Transcript:
RefSeq NM_022551.2 (NON-CURRENT)
Match location:
Position 166 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290966)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCATGTGGTGTTGAGGAAA
Hairpin Sequence:
5'-CCGG-GCTCATGTGGTGTTGAGGAAA-CTCGAG-TTTCCTCAACACCACATGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCATGTGGTGTTGAGGAAACTCGAGTTTCCTCAACACCACATGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCATGTGGTGTTGAGGAAACTCGAGTTTCCTCAACACCACATGAGC-3'

Other clones with same target sequence:

  1. TRCN0000074898