Construct: shRNA TRCN0000291011

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002047.2-694s21c1
DNA Barcode:
CAGCCCAAAGATGATATTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GARS1 (2617)
Gene Description:
glycyl-tRNA synthetase 1
Transcript:
RefSeq NM_002047.2 (NON-CURRENT)
Match location:
Position 694 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291011)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCCCAAAGATGATATTGTA
Hairpin Sequence:
5'-CCGG-CAGCCCAAAGATGATATTGTA-CTCGAG-TACAATATCATCTTTGGGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCCCAAAGATGATATTGTACTCGAGTACAATATCATCTTTGGGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCCCAAAGATGATATTGTACTCGAGTACAATATCATCTTTGGGCTG-3'

Other clones with same target sequence:

  1. TRCN0000045797