Construct: shRNA TRCN0000291234

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004078.2-679s21c1
DNA Barcode:
GCGAGATTTACTGCAAAGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSRP1 (1465)
Gene Description:
cysteine and glycine rich protein 1
Transcript:
RefSeq NM_004078.2 (NON-CURRENT)
Match location:
Position 679 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291234)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAGATTTACTGCAAAGGAT
Hairpin Sequence:
5'-CCGG-GCGAGATTTACTGCAAAGGAT-CTCGAG-ATCCTTTGCAGTAAATCTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAGATTTACTGCAAAGGATCTCGAGATCCTTTGCAGTAAATCTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAGATTTACTGCAAAGGATCTCGAGATCCTTTGCAGTAAATCTCGC-3'

Other clones with same target sequence:

  1. TRCN0000118078