Construct: shRNA TRCN0000291296

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022467.3-1424s21c1
DNA Barcode:
CCAGCACAACACCGTCCACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHST8 (64377)
Gene Description:
carbohydrate sulfotransferase 8
Transcript:
RefSeq NM_022467.3 (CURRENT)
Match location:
Position 1424 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291296)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCACAACACCGTCCACTA
Hairpin Sequence:
5'-CCGG-CCAGCACAACACCGTCCACTA-CTCGAG-TAGTGGACGGTGTTGTGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCACAACACCGTCCACTACTCGAGTAGTGGACGGTGTTGTGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCACAACACCGTCCACTACTCGAGTAGTGGACGGTGTTGTGCTGG-3'

Other clones with same target sequence:

  1. TRCN0000035098