Construct: shRNA TRCN0000291366

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006444.2-3238s21c1
DNA Barcode:
GCTATGAATGTATTGACAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMC2 (10592)
Gene Description:
structural maintenance of chromosomes 2
Transcript:
RefSeq NM_006444.2 (NON-CURRENT)
Match location:
Position 3238 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291366)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATGAATGTATTGACAGAA
Hairpin Sequence:
5'-CCGG-GCTATGAATGTATTGACAGAA-CTCGAG-TTCTGTCAATACATTCATAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATGAATGTATTGACAGAACTCGAGTTCTGTCAATACATTCATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATGAATGTATTGACAGAACTCGAGTTCTGTCAATACATTCATAGC-3'

Other clones with same target sequence:

  1. TRCN0000062542