Construct: shRNA TRCN0000291383

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001294.1-501s21c1
DNA Barcode:
CTCCATCTACATCCACGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLPTM1 (1209)
Gene Description:
CLPTM1 regulator of GABA type A receptor forward trafficking
Transcript:
RefSeq NM_001294.1 (NON-CURRENT)
Match location:
Position 501 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291383)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCATCTACATCCACGTTTA
Hairpin Sequence:
5'-CCGG-CTCCATCTACATCCACGTTTA-CTCGAG-TAAACGTGGATGTAGATGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCATCTACATCCACGTTTACTCGAGTAAACGTGGATGTAGATGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCATCTACATCCACGTTTACTCGAGTAAACGTGGATGTAGATGGAG-3'

Other clones with same target sequence:

  1. TRCN0000082947