Construct: shRNA TRCN0000291440

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006886.2-205s21c1
DNA Barcode:
GACTTCTGGCAGCAACGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP5F1E (514)
Gene Description:
ATP synthase F1 subunit epsilon
Transcript:
RefSeq NM_006886.2 (NON-CURRENT)
Match location:
Position 205 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291440)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTTCTGGCAGCAACGTAAA
Hairpin Sequence:
5'-CCGG-GACTTCTGGCAGCAACGTAAA-CTCGAG-TTTACGTTGCTGCCAGAAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTTCTGGCAGCAACGTAAACTCGAGTTTACGTTGCTGCCAGAAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTTCTGGCAGCAACGTAAACTCGAGTTTACGTTGCTGCCAGAAGTC-3'

Other clones with same target sequence:

  1. TRCN0000038216