Construct: shRNA TRCN0000291586

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017946.2-661s21c1
DNA Barcode:
GCGGTGGTGAATGAAAGTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FKBP14 (55033)
Gene Description:
FKBP prolyl isomerase 14
Transcript:
RefSeq NM_017946.2 (NON-CURRENT)
Match location:
Position 661 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291586)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGTGGTGAATGAAAGTCAT
Hairpin Sequence:
5'-CCGG-GCGGTGGTGAATGAAAGTCAT-CTCGAG-ATGACTTTCATTCACCACCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGTGGTGAATGAAAGTCATCTCGAGATGACTTTCATTCACCACCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGTGGTGAATGAAAGTCATCTCGAGATGACTTTCATTCACCACCGC-3'

Other clones with same target sequence:

  1. TRCN0000055899