Construct: shRNA TRCN0000291638

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006287.4-685s21c1
DNA Barcode:
TCCTGGAATATGTCGAGGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TFPI (7035)
Gene Description:
tissue factor pathway inhibitor
Transcript:
RefSeq NM_006287.4 (NON-CURRENT)
Match location:
Position 685 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291638)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTGGAATATGTCGAGGTTA
Hairpin Sequence:
5'-CCGG-TCCTGGAATATGTCGAGGTTA-CTCGAG-TAACCTCGACATATTCCAGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTGGAATATGTCGAGGTTACTCGAGTAACCTCGACATATTCCAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTGGAATATGTCGAGGTTACTCGAGTAACCTCGACATATTCCAGGA-3'

Other clones with same target sequence:

  1. TRCN0000073586