Construct: shRNA TRCN0000291666

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012405.3-476s21c1
DNA Barcode:
GCTGCTCTTTCTTCTTGGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ICMT (23463)
Gene Description:
isoprenylcysteine carboxyl methyltransferase
Transcript:
RefSeq NM_012405.3 (NON-CURRENT)
Match location:
Position 476 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291666)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTCTTTCTTCTTGGTTA
Hairpin Sequence:
5'-CCGG-GCTGCTCTTTCTTCTTGGTTA-CTCGAG-TAACCAAGAAGAAAGAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTCTTTCTTCTTGGTTACTCGAGTAACCAAGAAGAAAGAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTCTTTCTTCTTGGTTACTCGAGTAACCAAGAAGAAAGAGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000035269