Construct: shRNA TRCN0000291697

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024322.1-342s21c1
DNA Barcode:
GCCAGCGTGAAAGCATGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CENPO (79172)
Gene Description:
centromere protein O
Transcript:
RefSeq NM_024322.1 (NON-CURRENT)
Match location:
Position 342 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291697)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGCGTGAAAGCATGTATT
Hairpin Sequence:
5'-CCGG-GCCAGCGTGAAAGCATGTATT-CTCGAG-AATACATGCTTTCACGCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGCGTGAAAGCATGTATTCTCGAGAATACATGCTTTCACGCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGCGTGAAAGCATGTATTCTCGAGAATACATGCTTTCACGCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000072754