Construct: shRNA TRCN0000291793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025158.3-2360s21c1
DNA Barcode:
GCTCTGGTTCAGATACAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RUFY1 (80230)
Gene Description:
RUN and FYVE domain containing 1
Transcript:
RefSeq NM_025158.3 (NON-CURRENT)
Match location:
Position 2360 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGGTTCAGATACAACTT
Hairpin Sequence:
5'-CCGG-GCTCTGGTTCAGATACAACTT-CTCGAG-AAGTTGTATCTGAACCAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGGTTCAGATACAACTTCTCGAGAAGTTGTATCTGAACCAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGGTTCAGATACAACTTCTCGAGAAGTTGTATCTGAACCAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000073073