Construct: shRNA TRCN0000291834

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004265.2-923s21c1
DNA Barcode:
CCTACAATCACCAGCACGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FADS2 (9415)
Gene Description:
fatty acid desaturase 2
Transcript:
RefSeq NM_004265.2 (NON-CURRENT)
Match location:
Position 923 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291834)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACAATCACCAGCACGAAT
Hairpin Sequence:
5'-CCGG-CCTACAATCACCAGCACGAAT-CTCGAG-ATTCGTGCTGGTGATTGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACAATCACCAGCACGAATCTCGAGATTCGTGCTGGTGATTGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACAATCACCAGCACGAATCTCGAGATTCGTGCTGGTGATTGTAGG-3'

Other clones with same target sequence:

  1. TRCN0000064756