Construct: shRNA TRCN0000291874

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032268.4-1120s21c1
DNA Barcode:
CGAGATGGAAATGCACTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNRF1 (84937)
Gene Description:
zinc and ring finger 1
Transcript:
RefSeq NM_032268.4 (NON-CURRENT)
Match location:
Position 1120 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291874)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGATGGAAATGCACTTTAT
Hairpin Sequence:
5'-CCGG-CGAGATGGAAATGCACTTTAT-CTCGAG-ATAAAGTGCATTTCCATCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGATGGAAATGCACTTTATCTCGAGATAAAGTGCATTTCCATCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGATGGAAATGCACTTTATCTCGAGATAAAGTGCATTTCCATCTCG-3'

Other clones with same target sequence:

  1. TRCN0000073045