Construct: shRNA TRCN0000291950

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014018.2-317s21c1
DNA Barcode:
ACTGGTCATTGGACGGATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRPS28 (28957)
Gene Description:
mitochondrial ribosomal protein S28
Transcript:
RefSeq NM_014018.2 (NON-CURRENT)
Match location:
Position 317 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291950)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGGTCATTGGACGGATCTT
Hairpin Sequence:
5'-CCGG-ACTGGTCATTGGACGGATCTT-CTCGAG-AAGATCCGTCCAATGACCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGGTCATTGGACGGATCTTCTCGAGAAGATCCGTCCAATGACCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGGTCATTGGACGGATCTTCTCGAGAAGATCCGTCCAATGACCAGT-3'

Other clones with same target sequence:

  1. TRCN0000072615