Construct: shRNA TRCN0000291965

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000983.3-98s21c1
DNA Barcode:
GTTCTGAAGTTCACTCTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPL22 (6146)
Gene Description:
ribosomal protein L22
Transcript:
RefSeq NM_000983.3 (NON-CURRENT)
Match location:
Position 98 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291965)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCTGAAGTTCACTCTTGAT
Hairpin Sequence:
5'-CCGG-GTTCTGAAGTTCACTCTTGAT-CTCGAG-ATCAAGAGTGAACTTCAGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCTGAAGTTCACTCTTGATCTCGAGATCAAGAGTGAACTTCAGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCTGAAGTTCACTCTTGATCTCGAGATCAAGAGTGAACTTCAGAAC-3'

Other clones with same target sequence:

  1. TRCN0000075015