Construct: shRNA TRCN0000291969

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016207.2-1240s21c1
DNA Barcode:
CCAGTGAATTTATTCGTGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CPSF3 (51692)
Gene Description:
cleavage and polyadenylation specific factor 3
Transcript:
RefSeq NM_016207.2 (NON-CURRENT)
Match location:
Position 1240 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291969)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGAATTTATTCGTGCTT
Hairpin Sequence:
5'-CCGG-CCAGTGAATTTATTCGTGCTT-CTCGAG-AAGCACGAATAAATTCACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGAATTTATTCGTGCTTCTCGAGAAGCACGAATAAATTCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGAATTTATTCGTGCTTCTCGAGAAGCACGAATAAATTCACTGG-3'

Other clones with same target sequence:

  1. TRCN0000074985