Construct: shRNA TRCN0000291992

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001497.3-1060s21c1
DNA Barcode:
GCTCTAAGTAAACAACAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
B4GALT1 (2683)
Gene Description:
beta-1,4-galactosyltransferase 1
Transcript:
RefSeq NM_001497.3 (CURRENT)
Match location:
Position 1060 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000291992)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTAAGTAAACAACAGTTT
Hairpin Sequence:
5'-CCGG-GCTCTAAGTAAACAACAGTTT-CTCGAG-AAACTGTTGTTTACTTAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTAAGTAAACAACAGTTTCTCGAGAAACTGTTGTTTACTTAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTAAGTAAACAACAGTTTCTCGAGAAACTGTTGTTTACTTAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000034839