Construct: shRNA TRCN0000292015

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006159.2-2058s21c1
DNA Barcode:
GCTTCAATTTGGATGGCGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NELL2 (4753)
Gene Description:
neural EGFL like 2
Transcript:
RefSeq NM_006159.2 (CURRENT)
Match location:
Position 2058 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000292015)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCAATTTGGATGGCGGAT
Hairpin Sequence:
5'-CCGG-GCTTCAATTTGGATGGCGGAT-CTCGAG-ATCCGCCATCCAAATTGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCAATTTGGATGGCGGATCTCGAGATCCGCCATCCAAATTGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCAATTTGGATGGCGGATCTCGAGATCCGCCATCCAAATTGAAGC-3'

Other clones with same target sequence:

  1. TRCN0000054312