Construct: shRNA TRCN0000292045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006810.2-1335s21c1
DNA Barcode:
CCACACTGTAAGAAGGTCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDIA5 (10954)
Gene Description:
protein disulfide isomerase family A member 5
Transcript:
RefSeq NM_006810.2 (NON-CURRENT)
Match location:
Position 1335 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000292045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACACTGTAAGAAGGTCATT
Hairpin Sequence:
5'-CCGG-CCACACTGTAAGAAGGTCATT-CTCGAG-AATGACCTTCTTACAGTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACACTGTAAGAAGGTCATTCTCGAGAATGACCTTCTTACAGTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACACTGTAAGAAGGTCATTCTCGAGAATGACCTTCTTACAGTGTGG-3'

Other clones with same target sequence:

  1. TRCN0000049401