Construct: shRNA TRCN0000292064

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003725.2-774s21c1
DNA Barcode:
CGAATGAAGCAAAGTTGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HSD17B6 (8630)
Gene Description:
hydroxysteroid 17-beta dehydrogenase 6
Transcript:
RefSeq NM_003725.2 (NON-CURRENT)
Match location:
Position 774 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000292064)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAATGAAGCAAAGTTGGAAA
Hairpin Sequence:
5'-CCGG-CGAATGAAGCAAAGTTGGAAA-CTCGAG-TTTCCAACTTTGCTTCATTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAATGAAGCAAAGTTGGAAACTCGAGTTTCCAACTTTGCTTCATTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAATGAAGCAAAGTTGGAAACTCGAGTTTCCAACTTTGCTTCATTCG-3'

Other clones with same target sequence:

  1. TRCN0000049472