Construct: shRNA TRCN0000292095

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015529.2-606s21c1
DNA Barcode:
GCGGTTATTGAATCCTGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MOXD1 (26002)
Gene Description:
monooxygenase DBH like 1
Transcript:
RefSeq NM_015529.2 (NON-CURRENT)
Match location:
Position 606 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000292095)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGTTATTGAATCCTGAGAA
Hairpin Sequence:
5'-CCGG-GCGGTTATTGAATCCTGAGAA-CTCGAG-TTCTCAGGATTCAATAACCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGTTATTGAATCCTGAGAACTCGAGTTCTCAGGATTCAATAACCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGTTATTGAATCCTGAGAACTCGAGTTCTCAGGATTCAATAACCGC-3'

Other clones with same target sequence:

  1. TRCN0000078176