Construct: shRNA TRCN0000292162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018404.2-1883s21c1
DNA Barcode:
TGAAAGATAAAGTAGTCAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADAP2 (55803)
Gene Description:
ArfGAP with dual PH domains 2
Transcript:
RefSeq NM_018404.2 (NON-CURRENT)
Match location:
Position 1883 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000292162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAAGATAAAGTAGTCAATG
Hairpin Sequence:
5'-CCGG-TGAAAGATAAAGTAGTCAATG-CTCGAG-CATTGACTACTTTATCTTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAAGATAAAGTAGTCAATGCTCGAGCATTGACTACTTTATCTTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAAGATAAAGTAGTCAATGCTCGAGCATTGACTACTTTATCTTTCA-3'

Other clones with same target sequence:

(none)