Construct: shRNA TRCN0000292256

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025065.6-453s21c1
DNA Barcode:
CAACAAACAGACTTCTCCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPF1 (80135)
Gene Description:
ribosome production factor 1 homolog
Transcript:
RefSeq NM_025065.6 (NON-CURRENT)
Match location:
Position 453 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000292256)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACAAACAGACTTCTCCCAA
Hairpin Sequence:
5'-CCGG-CAACAAACAGACTTCTCCCAA-CTCGAG-TTGGGAGAAGTCTGTTTGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACAAACAGACTTCTCCCAACTCGAGTTGGGAGAAGTCTGTTTGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACAAACAGACTTCTCCCAACTCGAGTTGGGAGAAGTCTGTTTGTTG-3'

Other clones with same target sequence:

  1. TRCN0000154061