Construct: shRNA TRCN0000292356

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006809.4-1857s21c1
DNA Barcode:
CCGGGCTTCATAGTTCTTTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TOMM34 (10953)
Gene Description:
translocase of outer mitochondrial membrane 34
Transcript:
RefSeq NM_006809.4 (NON-CURRENT)
Match location:
Position 1857 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000292356)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGGCTTCATAGTTCTTTGT
Hairpin Sequence:
5'-CCGG-CCGGGCTTCATAGTTCTTTGT-CTCGAG-ACAAAGAACTATGAAGCCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGGCTTCATAGTTCTTTGTCTCGAGACAAAGAACTATGAAGCCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGGCTTCATAGTTCTTTGTCTCGAGACAAAGAACTATGAAGCCCGG-3'

Other clones with same target sequence:

  1. TRCN0000166639