Construct: shRNA TRCN0000292443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004427.3-1572s21c1
DNA Barcode:
GCTTCCTCCAAAGTCCCAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHC2 (1912)
Gene Description:
polyhomeotic homolog 2
Transcript:
RefSeq NM_004427.3 (NON-CURRENT)
Match location:
Position 1572 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000292443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCCTCCAAAGTCCCAATA
Hairpin Sequence:
5'-CCGG-GCTTCCTCCAAAGTCCCAATA-CTCGAG-TATTGGGACTTTGGAGGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCCTCCAAAGTCCCAATACTCGAGTATTGGGACTTTGGAGGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCCTCCAAAGTCCCAATACTCGAGTATTGGGACTTTGGAGGAAGC-3'

Other clones with same target sequence:

  1. TRCN0000166083