Construct: shRNA TRCN0000292498

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016274.4-560s21c1
DNA Barcode:
GTTTACTCTTGCCCACTCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLEKHO1 (51177)
Gene Description:
pleckstrin homology domain containing O1
Transcript:
RefSeq NM_016274.4 (NON-CURRENT)
Match location:
Position 560 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000292498)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTACTCTTGCCCACTCCAA
Hairpin Sequence:
5'-CCGG-GTTTACTCTTGCCCACTCCAA-CTCGAG-TTGGAGTGGGCAAGAGTAAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTACTCTTGCCCACTCCAACTCGAGTTGGAGTGGGCAAGAGTAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTACTCTTGCCCACTCCAACTCGAGTTGGAGTGGGCAAGAGTAAAC-3'

Other clones with same target sequence:

  1. TRCN0000165209