Construct: shRNA TRCN0000292613

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026502.1-989s21c1
DNA Barcode:
CCTCAACCCACCAAATTCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1110004E09Rik (68001)
Gene Description:
RIKEN cDNA 1110004E09 gene
Transcript:
RefSeq NM_026502.1 (NON-CURRENT)
Match location:
Position 989 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000292613)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAACCCACCAAATTCCTA
Hairpin Sequence:
5'-CCGG-CCTCAACCCACCAAATTCCTA-CTCGAG-TAGGAATTTGGTGGGTTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAACCCACCAAATTCCTACTCGAGTAGGAATTTGGTGGGTTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAACCCACCAAATTCCTACTCGAGTAGGAATTTGGTGGGTTGAGG-3'

Other clones with same target sequence:

  1. TRCN0000184159