Construct: shRNA TRCN0000292672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026353.3-272s21c1
DNA Barcode:
CTTGGTGACTCACGTGATGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc48a1 (67739)
Gene Description:
solute carrier family 48 (heme transporter), member 1
Transcript:
RefSeq NM_026353.3 (NON-CURRENT)
Match location:
Position 272 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000292672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGGTGACTCACGTGATGTA
Hairpin Sequence:
5'-CCGG-CTTGGTGACTCACGTGATGTA-CTCGAG-TACATCACGTGAGTCACCAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGGTGACTCACGTGATGTACTCGAGTACATCACGTGAGTCACCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGGTGACTCACGTGATGTACTCGAGTACATCACGTGAGTCACCAAG-3'

Other clones with same target sequence:

  1. TRCN0000201891