Construct: shRNA TRCN0000292683

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172257.3-1906s21c1
DNA Barcode:
GCAGCGAGAGATCAATCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sidt2 (214597)
Gene Description:
SID1 transmembrane family, member 2
Transcript:
RefSeq NM_172257.3 (NON-CURRENT)
Match location:
Position 1906 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000292683)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCGAGAGATCAATCATAA
Hairpin Sequence:
5'-CCGG-GCAGCGAGAGATCAATCATAA-CTCGAG-TTATGATTGATCTCTCGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCGAGAGATCAATCATAACTCGAGTTATGATTGATCTCTCGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCGAGAGATCAATCATAACTCGAGTTATGATTGATCTCTCGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000175700