Construct: shRNA TRCN0000292715

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_171826.2-1158s21c1
DNA Barcode:
GCAGTAACTATGAAACTAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cldnd1 (224250)
Gene Description:
claudin domain containing 1
Transcript:
RefSeq NM_171826.2 (NON-CURRENT)
Match location:
Position 1158 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000292715)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTAACTATGAAACTAGAA
Hairpin Sequence:
5'-CCGG-GCAGTAACTATGAAACTAGAA-CTCGAG-TTCTAGTTTCATAGTTACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTAACTATGAAACTAGAACTCGAGTTCTAGTTTCATAGTTACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTAACTATGAAACTAGAACTCGAGTTCTAGTTTCATAGTTACTGC-3'

Other clones with same target sequence:

  1. TRCN0000174740