Construct: shRNA TRCN0000292833

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008477.2-2933s21c1
DNA Barcode:
CCCTCTCAGGAAGAATTACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ktn1 (16709)
Gene Description:
kinectin 1
Transcript:
RefSeq NM_008477.2 (CURRENT)
Match location:
Position 2933 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000292833)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCTCAGGAAGAATTACAA
Hairpin Sequence:
5'-CCGG-CCCTCTCAGGAAGAATTACAA-CTCGAG-TTGTAATTCTTCCTGAGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCTCAGGAAGAATTACAACTCGAGTTGTAATTCTTCCTGAGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCTCAGGAAGAATTACAACTCGAGTTGTAATTCTTCCTGAGAGGG-3'

Other clones with same target sequence:

  1. TRCN0000173131