Construct: shRNA TRCN0000293008

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172599.3-605s21c1
DNA Barcode:
AGAGCTCACCTCCATCATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atg14 (218978)
Gene Description:
VATG14 autophagy related 14 homolog (S. cerevisiae)
Transcript:
RefSeq NM_172599.3 (NON-CURRENT)
Match location:
Position 605 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293008)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGCTCACCTCCATCATATT
Hairpin Sequence:
5'-CCGG-AGAGCTCACCTCCATCATATT-CTCGAG-AATATGATGGAGGTGAGCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGCTCACCTCCATCATATTCTCGAGAATATGATGGAGGTGAGCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGCTCACCTCCATCATATTCTCGAGAATATGATGGAGGTGAGCTCT-3'

Other clones with same target sequence:

(none)