Construct: shRNA TRCN0000293096

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146078.2-5448s21c1
DNA Barcode:
CCAGAAAGACAACAGGAAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ubr2 (224826)
Gene Description:
ubiquitin protein ligase E3 component n-recognin 2
Transcript:
RefSeq NM_146078.2 (NON-CURRENT)
Match location:
Position 5448 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293096)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAAAGACAACAGGAAATA
Hairpin Sequence:
5'-CCGG-CCAGAAAGACAACAGGAAATA-CTCGAG-TATTTCCTGTTGTCTTTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAAAGACAACAGGAAATACTCGAGTATTTCCTGTTGTCTTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAAAGACAACAGGAAATACTCGAGTATTTCCTGTTGTCTTTCTGG-3'

Other clones with same target sequence:

  1. TRCN0000175322