Construct: shRNA TRCN0000293202

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005625.3-399s21c1
DNA Barcode:
AGCAAGACCTTCCAGTATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SDCBP (6386)
Gene Description:
syndecan binding protein
Transcript:
RefSeq NM_005625.3 (NON-CURRENT)
Match location:
Position 399 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293202)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAAGACCTTCCAGTATAAA
Hairpin Sequence:
5'-CCGG-AGCAAGACCTTCCAGTATAAA-CTCGAG-TTTATACTGGAAGGTCTTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAAGACCTTCCAGTATAAACTCGAGTTTATACTGGAAGGTCTTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAAGACCTTCCAGTATAAACTCGAGTTTATACTGGAAGGTCTTGCT-3'

Other clones with same target sequence:

(none)